Mythili M Iyer was initiated into music by her first guru Sri. S V Ramakrishnan and later on she had an opportunity to take advanced training under senior musicians like Sri. V R Krishnan and Sri P S Narayanaswami (disciples of Sri Semmangudi Srinivasa Iyer) and Sri D K Jayaraman. Smt Mythili Iyer has a Master's degree in Carnatic Music from Madras University and was teaching music at Sitalakshmi Ramaswami College in Tiruchirapalli in her early years. Mythili has served as an All India Radio artiste in Mumbai, where she gave a number of public concerts. She has given a number of concerts in Sydney in the last 15 years and is teaching Carnatic Music to several students.